PJB-2021-1531
In-silico and phylogenetic analysis of DREB transcription factor in Solanum melongena L.
Amna Ahmed, Syeda Qamarunnisa, Ishrat Jamil, Saboohi Raza and Khalida Khatoon
Abstract
Solanum melongena L. belongs to family Solanaceae is an agronomically, economically and medicinally important crop plant. The crop is sensitive to severe environmental conditions thus open the door to explore its genome for possible genetic improvement. Abiotic stresses are well known to adversely affect crop productivity and yield. Dehydration responsive element binding (DREB) proteins are reported to play a significant role in abiotic stress tolerance. Solanum melongena genome was searched for the presence of DREB1 gene and structural-functional analyses were carried out by using different in silico, bioinformatics and phylogenetics tools. It was observed that SmDREB1 showed 92% and 81% sequence similarity at nucleotide and protein level respectively with Solanum tuberosum DREB1. Most of the short linear motifs harbored by SmDREB1 were also seen conserved in StDREB1 however, six motifs were found only in SmDREB1 which make this protein a good candidate to be used in crop engineering approaches. The translated protein has three intrinsically disordered regions, which include 89.5% of total amino acids. This feature categorizes SmDREB1 under unstructured protein that is an attribute of transcription factors. The 3D structure prediction presented nine residues mostly tyrosine and arginine involved in binding with nucleic acid. Bayesian based phylogenetic analysis inferred that DREB1 from S. melongena is a close homologue of DREB1 from other Solanum species although it may perform some additional biological functions. The results of this study highlighted the importance of SmDREB1 that could be a good candidate gene for the incorporation of abiotic stress tolerance in crop plants.
To Cite this article:
Ahmed, A., S. Qamarunnisa, I. Jamil, S. Raza and K. Khatoon. 2021. In-silico and phylogenetic analysis of DREB transcription factor in Solanum melongena L.. Pak. J. Bot., 53(4): DOI: http://dx.doi.org/10.30848/PJB2021-4(2)
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