PJB-2021-269
Role of mycorrhizospheric fluorescent Pseudomonas in suppressing the root rot disease, enhancement of vesicular arbuscular mycorrhizal (VAM) population and phosphorus uptake in sunflower
Syeda Sadia Bokhari, Hafiza Farhat, Syed Abid Ali, Faizah Urooj, Afshan Rahman, Jehan Ara, Muhammed Irfan and Syed Ehteshamul-Haque
Abstract
The vesicular arbuscular mycorrhizal (VAM) symbiosis is known to improve nutrient uptake by plants particularly phosphorus, and suppress soilborne plant pathogens. However, mycorrhizospheric bacteria also affect VAM fungi and their host plant. A total of 87 isolates of fluorescent Pseudomonas (MRFP) were isolated from the mycorrhizosphere of 15 plant species in this study. These isolates were initially identified on the basis of biochemical tests. Molecular biology tools (16S rDNA gene sequencing) were used to confirm the identification of promising isolates. These isolates were evaluated against root rot pathogens, Macrophomina phaseolina, Fusarium solani, F.oxysporum and Rhizoctonia solani and Meloidogyne javanica, a root knot nematode in vitro, suppressed growth of most of the fungi and demonstrated strong nematicidal activity. Performance of promising isolates of MRFP were evaluated on sunflower in pots and field plot experiments showed significant suppressive effect on root rot pathogens resulting in the production of taller plants having greater shoot weight and flower weight as compared to control plants. Application of MRFP both in pots and field plot experiments significantly increased VAM population around roots and generally improved phosphorus uptake by plants. MRFP was found very effective in ameliorating activity of native VAM. It seems that MRFP plays some role in the stimulation and proliferation of VAM fungi in plant-fungus interaction
To Cite this article:
Bokhari, S.S., H. Farhat, S.A. Ali, F. Urooj, A. Rahman, J. Ara, M. Irfan and S.E. Haque. 2023. Role of mycorrhizospheric fluorescent Pseudomonas in suppressing the root rot disease, enhancement of vesicular arbuscular mycorrhizal (VAM) population and phosphorus uptake in sunflower. Pak. J. Bot., 55(2): DOI: http://dx.doi.org/10.30848/PJB2023-2(36)
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