PJB-2021-439
Development of a micropropagation protocol for endangered Hypericum bilgehan-bilgilii Başkose & Savran (Hypericaceae) species, local endemic to Turkey
Huseyin Turker and Bengu Turkyilmaz Unal
Abstract
Hypericum species, are medicinally important and well studied for their pharmaceutical values. These species are gradually losing ground due to habitat loss, serious anthropogenic reasons as well as excessive collection from nature. Hypericum bilgehan-bilgilii is a local endemic species in Turkey. The population size is small and facing a threat. Keeping this in view there was a need to develop an In vitro ex-situ conservation protocol. Therefore an attempt was made to develop a protocol for callus induction, shoot growth, rooting and acclimatization for this endemic species. The nodal parts of the plant were preferred as the source of explant. These were cultured on a MS medium containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and Kinetin (Kin) in different concentrations and combinations. The best callus induction was recorded in MS medium including 1.0 mg.L-1 Kin and 0.5 mg.L-1 2,4-D with 6.2 shoots per callus. MS media with different Kin concentrations were used for shoot growth. The best shoot growth was observed on the MS medium with 2.0 mg.L-1 Kin. Shoots grown In vitro were subcultured to MS medium containing 0.5 and 1.0 mg.L-1 indole-3-butyric acid (IBA) for rooting. The best-rooting frequency (90 %) and rooting were determined in MS medium containing 1.0 mg.L-1 IBA. There were 10 root formations per shoot. The micropropagated plantlets were acclimatized in pots filled with peat and by this way, an effective In vitro tissue culture protocol for the species studied was developed and its sustainable conservation way was confirmed
To Cite this article:
Turker, H. and B.T. Unal. 2022. Development of a micropropagation protocol for endangered Hypericum bilgehan-bilgilii Başkose & Savran (Hypericaceae) species, local endemic to Turkey. Pak. J. Bot., 54(3): DOI: http://dx.doi.org/10.30848/PJB2022-3(44)
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