PJB-2021-440
Evaluation of the role of different types of cytokinins as zeatin replacement in in vitro proliferation of Vaccinium dunalianum
Dakuan Sun, Mengting Wang, Ping Zhao, Yong Ding, Biyou Huang and Junrong Tang
Abstract
In plant tissue culture, cytokinin is an important limiting factor for rapid proliferation, but the effects of different types of phytokinins on the proliferation of different plants vary. Isoprenoid cytokinin is more valid for shoot initiation in Vaccinium species, the purpose of our research was to study whether Vaccinium dunalianum has a preference for Zeatin. V. dunalianum, which belongs to Ericaceae, is rich in caffeoylarbutin derivatives. In the early stage, the proliferation system of V. dunalianum was established using zeatin (ZT). In this study, V. dunalianum was used as experimental material to evaluate the effects of substituting kinetin (Kin), 6-benzylaminopurine (BA), or thidiazuron (TDZ) for ZT in the shoot proliferation stage of V. dunalianum. The proliferation rate of V. dunalianum decreased when ZT was completely replaced by the other types of cytokinins. When ZT was partially replaced by Kin, 2.0 mg∙L-1 Kin and 0.5 mg∙L-1 ZT was discovered to be the optimal combination, and the effective multiplication rate was 3.40. When ZT was partially replaced by BA, 1.0 mg∙L-1 BA and 0.5 mg∙L-1 ZT was the best combination, and the effective multiplication rate was 2.26. When ZT was partially replaced by TDZ, malformed buds appeared. Of the combinations tested, 1.0 mg∙L-1 Kin, 1.0 mg∙L-1 BA, and 0.1 mg∙L-1 ZT led to the greatest shoot proliferation rate (4.32), and the growth was normal. In conclusion, ZT is preferred for shoot multiplication of V. dunalianum. When ZT was added to the medium, the propagation of V. dunalianum was easier and the growth of seedlings was better. Results could provide technical support for reducing the production cost of tissue culture seedlings of V. dunalianum and a certain reference for selection of tissue culture formulas of other Vaccinium plants
To Cite this article:
Sun, D., M. Wang, P. Zhao, Y. Ding, B. Huang and J. Tang. 2023. Evaluation of the role of different types of cytokinins as zeatin replacement in in vitro proliferation of Vaccinium dunalianum. Pak. J. Bot., 55(1): DOI: http://dx.doi.org/10.30848/PJB2023-1(25)
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