PJB-2021-456
Identification and association of NBS-LRR encoding disease resistance gene analogs family in diploid cotton (Gossypium arboreum L.)
Sajjad Haidar, Shahid Mansoor and Iftikhar Ahmad Khan
Abstract
Identification of genes at molecular level and association as linked markers to resistance in diploid cotton with emphasis on major diseases of cotton was studied via a conserved domain strategy by polymerase chain reaction (PCR). Three rounds of PCR amplifications by using degenerate oligonucleotide primers corresponding to the conserved motifs, nucleotide binding sites (NBS) and leucine rich repeats (LRRs) were performed. According to the primer wise expected PCR products size, forty nine required size products amplified were eluted and some were directly precipitated from PCR reactions. The identified eluted products were cloned and sequenced. Through this approach resistance genes analogs (RGA) like sequences were determined that are may be candidates for known disease resistance loci. All of the eluted identified candidate resistance genes loci’s (RGLs) are not RGAs. However some of the common types of equal size RGLs may be used as markers to identify the resistance genes. These results demonstrates that mapping of RGA sequences can be useful for landing markers linked to known resistance genes analogs and possibly in identifying candidate resistance loci in different species of cotton. Genome characterization for cotton leaf curl virus (CLCuV) study suggested that 800 and 1200 base pair size (bps) fragments could be used as markers for resistance to CLCuV. We named these markers as MCLCuV-1 and MCLCuV-2.
To Cite this article:
Haidar, S., S. Mansoor and I.A. Khan. 2023. Identification and association of NBS-LRR encoding disease resistance gene analogs family in diploid cotton (Gossypium arboreum L.). Pak. J. Bot., 55(6): DOI: http://dx.doi.org/10.30848/PJB2023-6(4)
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