PJB-2022-1516
Analysis of genetic diversity among local and exotic Pisum sativum genotypes through RAPD and SSR markers
Asif Javaid, Abdul Ghafoor and Malik Ashiq Rabbani
Abstract
Pea is an important temperate legume that is used as a vegetable and pulse crop. Study of genetic diversity in the crop using molecular markers helps to enhance the plant breeding efforts. The experiment was conducted to evaluate 88 pea germplasm accessions from the National Genebank of Pakistan that consisted of 61 landraces and 27 improved varieties from Pakistan and other countries. Random Amplified Polymorphic DNA (RAPD) analysis was conducted using 20 decamer oligonucleotide primers. Fifteen previously reported primers were used for Simple Sequence Repeat (SSR) analysis. Data was recorded for 100 polymorphic RAPD bands and 51 polymorphic SSR bands. Data analysis was conducted using statistical package NTSYSpc version 2.21q. Cluster analysis of germplasm using RAPD analysis revealed three major groups. First group included 11 landraces from Pakistan and one landrace from Afghanistan. This group was further divided into two sub-groups by SSR analysis. Accession of Afghanistan was separated from 11 accessions of Pakistan that remained in the same cluster. Clustering of 11 accessions from Pakistan and one accession from Afghanistan by RAPD analysis shows that this region has distinctive landraces as previously reported. Separation of landraces from Pakistan and Afghanistan by SSR shows that Pakistan has its indigenous landraces of Pisum sativum germplasm. Further studies should be conducted on pea landraces and wild relatives from Pakistan for their utilization in crop improvement.
To Cite this article:
Javaid, A., A. Ghafoor and M.A. Rabbani. 2022. Analysis of genetic diversity among local and exotic Pisum sativum genotypes through RAPD and SSR markers. Pak. J. Bot., 54(3): DOI: http://dx.doi.org/10.30848/PJB2022-3(18)
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