PJB-2021-620
Analysis of physiological indexes and aroma-related genes of thick-skinned melon (Cucumis melo L. var. Hetau) under salt stress
Liqiang Zhao, Zewei Zhang, Fengshuo Wang, Jingshu Li, Yuchen Liu, Yanli Ding, Guoze Wang and Jin Jia
Abstract
Comprehensive performance and aroma synthase in melon seedling under salt stress could directly affects the aromas content of its fruit. In the present research, when the height of the Cucumis melo L. cv Hetau was 10~15 cm, the seedlings were treated using Hoagland with 0, 50, 100, 150 and 200 mM NaCl. After three days of NaCl stress, the tender leaves of C. melo L. cv Hetau were cut for the extraction of total RNA, semi-quantitative PCR was used to analyze the lipoxygenase (LOX) and alcohol dehydrogenase 3 (ADH3) gene expression in C. melo L. cv Hetau under NaCl stress, and after 40 days, samples were taken for the determination of chlorophyll content, root activity based on α-naphthylamine content, nitrate reductase activities (NR), LOX activities and the alcohol dehydrogenase (ADH) activities in C. melo L. cv Hetau. The results showed that the relative expression of LOX and ADH3 genes increased with NaCl increase, and reached the highest level under 100 mM NaCl, the relative expression of the genes decreased under 150-200 mM NaCl. The chlorophyll content, root activity based on α-naphthylamine content and ADH activities in C. melo L. cv Hetau rose first, and then decreased with NaCl increase. The chlorophyll contents, root activities based on α-naphthylamine content and LOX activities reached the highest value under 50 mM NaCl, and ADH and NR activities reached the highest value at 100 mM NaCl. These results indicate that higher NaCl can obviously impact the physiological reaction of C. melo L. cv Hetau, and the impact is more obvious with the increase of NaCl. The study indicated that the moderate salt could adjust the aroma of muskmelon to the best
To Cite this article:
Zhao, L., Z. Zhang, F. Wang, J. Li, Y. Liu, Y. Ding, G. Wang and J. Jia. 2023. Analysis of physiological indexes and aroma-related genes of thick-skinned melon (Cucumis melo L. var. Hetau) under salt stress. Pak. J. Bot., 55(5): DOI: http://dx.doi.org/10.30848/PJB2023-5(28)
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