PJB-2021-750
Influence of plant growth regulators on the phenolic composition of Elaeocarpus grandiflorus J.E. Smith (Elaeocarpaceae) cell culture
Noor Aini Habibah, Nugrahaningsih Nugrahaningsih, Yustinus Ulung Anggraito, Safitri Safitri, Fajar Musafa and Nur Wijawati
Abstract
Elaeocarpus grandiflorus (Elaeocarpaceae) has the potential to be developed as an antidiabetic agent and as a source of antioxidants. However, massive exploration of this species is very risky and likely to be extinct because it is rare and difficult to cultivate. Cell culturing of E. grandiflorus is an effective alternative technique for producing secondary metabolites, especially phenolic acid. Picloram and 2,4-Dichlorophenoxyacetic acid (2,4-D) are plant growth regulators in the form of synthetic auxins which are widely used to increase the production of secondary metabolites in the cell culturing process. Therefore, this study aimed to analyze the effect of the type and concentration of picloram and 2,4-D on the composition of phenolic acids. The organ samples for the cell suspension culture were petioles of mature leaves obtained from two-year-old E. grandiflorus plants. Petiole was prepared aseptically and inoculated on a woody plant medium and induced using 2,4-D at a dose of 1.5 ppm, 2.5 ppm, and 3.5 ppm and picloram at a dose of 2.5 ppm, 5.0 ppm, and 7.5 ppm. Cultures were maintained for 35 days and then analyzed using liquid chromatography-mass spectrometry to measure the phenolic content. The results showed that picloram and 2,4-D with various concentrations had the same effect in inducing the formation of phenolic compounds in E. grandiflorus cell cultures. However, cell suspension cultures derived from the petiole of young leaves produced various types of phenolic acid compounds. The dominant compound found was kaempferol, which accounted for more than 16% of the total identified secondary metabolites. Further research is needed to determine the mechanism of action of picloram and 2,4-D in influencing the productivity of phenolic acid compounds
To Cite this article:
Habibah, N.A., N. Nugrahaningsih, Y.U. Anggraito, S. Safitri, F. Musafa and N. Wijawati. 2023. Influence of plant growth regulators on the phenolic composition of Elaeocarpus grandiflorus J.E. Smith (Elaeocarpaceae) cell culture. Pak. J. Bot., 55(5): DOI: http://dx.doi.org/10.30848/PJB2023-5(17)
Download
