PJB-2022-56
Cloning and expression profile of PdPapDOL3 gene in Shanxin poplar (Populus davidana × P. alba var. Pyramidlis) in response to stress
Yuan Yin, Abdul Majeed Baloch, Xiaoxuan Chen, Yida Che, Jiamin Lei, Yue Zhang, Surui Liu, Abdul Wahid Baloch and Rongshu Zhang
Abstract
In order to identify and verify crucial genes that regulate the stress response and resistance of poplar, RNA-seq data of poplar (Populus davidiana × P. alba var. Pyramidlis, cv ‘Shanxin’) leaves induced by Trichoderma asperellum and/or Alternaria alternata were screened. As a result, a key responding gene was identified, cloned and named as PdPapDOL3. In silico analyses showed that the coded protein PdPapDOL3 was a non-transmembrane hydrophilic protein of the DOG1 transcription factor family. The tissue-specific expression profile of PdPapDOL3 in poplar seedlings was investigated through real-time quantitative polymerase chain reaction (RT-qPCR). The results showed that PdPapDOL3 was constititutively expressed in different compartments of poplar with the root demonstrating the highest expression. The differential regulation of PdPapDOL3 expression induced by salt, alkali, polyethylene glycol (PEG), five soil-borne plant fungal pathogens or phytohormones for 48 hours was separately investigated. The results showed that the alkali induction caused the most evident change in PdPapDOL3 expression. Fusarium oxysporum, Cytospora chrysosperma or Alternaria alternata induction significantly up-regulated PdPapDOL3 expression in young tissues. F. oxysporum induction significantly up-regulated PdPapDOL3 expression in mature leaf. C. chrysosperma and A. alternata significantly induced PdPapDOL3 expression in the root. PdPapDOL3 expression was induced by salicylic acid (SA) in all the investigated compartments of poplar. While induced by jasmonic acid (JA) or abscisic acid (ABA), PdPapDOL3 expression was up-regulated in young tissues but down-regulated in the root. In this study, we report the tissue-specific expression patterns and the differential regulation profiles of the PdPapDOL3 gene in poplar under various stress conditions. Our results lay a foundation for further elucidating the function of PdPapDOL3 and provide insights into breeding novel stress-resistant poplar cultivars through modifying PdPapDOL3 expression
To Cite this article:
Yin, Y., A.M. Baloch, X. Chen, Y. Che, J. Lei, Y. Zhang, S. Liu, A.W. Baloch and R. Zhang. 2023. Cloning and expression profile of PdPapDOL3 gene in Shanxin poplar (Populus davidana × P. alba var. Pyramidlis) in response to stress. Pak. J. Bot., 55(2): DOI: http://dx.doi.org/10.30848/PJB2023-2(28)
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