PJB-2022-94
Callogenesis from Persea americana (Mill.) leaf tissues: a histological comparison of materials grown in greenhouse and in vitro
Rebecca O. Oyerinde and David J. Mycock
Abstract
Phenotypic plasticity has been recorded in cultivars that are grown under different environmental conditions. The present study assessed the morphological differences of callogenic leaf tissues from the greenhouse and the In vitro cultured plants of Persea americana cv ‘edranol’ (avocado) using light microscopy. Callus was formed from all tissues of the leaves and morphological observations indicated that In vitro materials were less differentiated and more meristematic than greenhouse materials with 80.6% and 72% callus formation, respectively. Further morphological observation of fresh leaves obtained from both sources showed significant differences. Cells of In vitro-derived leaves were bigger resulting in thicker leaves (171.19 ± 21.6 µm) than greenhouse-derived leaves (75.98 ± 8.6 µm). Bundle sheath extensions (BSE) were present in the greenhouse leaves but absent in the In vitro leaves. In vitro leaf cells contained fewer chloroplasts (1 chloroplast/100 μm2) than the cells of greenhouse leaves (3 chloroplast/100 μm2). This study established that growth environments could have significant impact on the callogenic competence of explants as well as influence the plant physiology of the parent plants
To Cite this article:
Oyerinde, R.O. and D.J. Mycock. 2024. Callogenesis from Persea americana (Mill.) leaf tissues: a histological comparison of materials grown in greenhouse and in vitro. Pak. J. Bot., 56(4): DOI: http://dx.doi.org/10.30848/PJB2024-4(37)
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