PJB-2022-463
Synergistic effect of Citrus sinensis (orange) and Aloe barbadensis (aloe vera) extracts on biosynthesis and antimicrobial activities of zinc oxide nanoparticles
Muhammad Asjad Khan, Matiullah Khan, Rehan Naeem, Maria Hafeez, Ume-Nasaria Manzoor, Yousra Anwar and Sadia
Abstract
The synthesis of nanoparticles through the green route has become a straightforward, protected, long-lasting, consistent, and ecologically sustainable process. Bacteria are increasing its resistance against antibacterial solutions which is becoming a very critical issue nowadays. Nanoparticles can help prevent the distribution of resistance in bacteria. Metallic nanoparticles like Zinc oxide nanoparticles (ZnONPs) are among the most promising agents because they showed strong antibacterial activity without any scarcity of resistance development inside the microbes. Synthesis of ZnONPs from Citrus sinensis and Aloe vera leaf extract was conducted in this study. This was performed with the concept that the medicinal effectiveness of NPs relies on their antibacterial features. Fourier Transform Infrared Spectroscopy (FTIR) analysis has revealed that availability of aromatic compounds, amides, phenolics, and alkaloids in the stabilization, capping, and bio-reduction of ZnONPs. Furthermore, X-Ray Diffraction Analysis estimated the crystalline structure of ZnONPs to be hexagonal, and the size was found to be 15.6 nm. Transmission Electron Microscopy (TEM) determined the shape of the nanoparticle as a mixed spherical-triangular shape. All these techniques were utilized to obtain the morphology and crystalline nature of nanoparticles. ZnONPs derived from green synthesis, demonstrated promising anti-bacterial activity against E. coli, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella, Methicillin-resistant Staphylococcus aureus. Green synthesized ZnONPs exhibited a maximum inhibition zone against Salmonella typhi (9.5 mm) and Methicillin-resistant Staphylococcus aureus (MRSA) (19.3 mm) and a minimum inhibition zone against E. coli (16 mm).
To Cite this article:
Khan, M.A., M. Khan, R. Naeem, M. Hafeez, U.N. Manzoor, Y. Anwar and Sadia. 2024. Synergistic effect of Citrus sinensis (orange) and Aloe barbadensis (aloe vera) extracts on biosynthesis and antimicrobial activities of zinc oxide nanoparticles. Pak. J. Bot., 56(4): DOI: http://dx.doi.org/10.30848/PJB2024-4(9)
Download
