PJB-2023-50
Barcoding of Asteraceae plants of juniper ecosystem Ziarat, Balochistan
Shazia Irfan, Shahjahan Shabbir Ahmed Rana, Muhammad Mushtaq, Imran Ali Sani, Razia Kanwal, Saadullah, Dawood Shahid, Rukhsana Jabeen and Nazeer Ahmed
Abstract
The Asteraceae family, which includes some economically and medicinally important plant species such as sunflower (Helianthus annus), salad leaves (Lactuca sativa) and Artemisia species has been a focus of DNA barcoding research due to the challenges in identifying these species using traditional morphological methods. The present study aimed to develop and evaluate the ability of the DNA barcodes targeting chloroplast genomic regions i.e., Riboluse-1,5- Biphosphate Carboxylase/ Oxygenase Large subunit (rbcL), and Maturase K (matK) for several plant species belonging to family Asteraceae collected from Juniper ecosystem Ziarat, Pakistan. The overall results indicated that both matK and rbcL markers successfully amplified the targeted regions and sequenced. During species resolution, matK (70% and 82%) showed better results compared to rbcL (42% and 48%) through BLASTN analysis and Neighbor Joining tree analysis respectively. Interspecific and Intraspecific divergence showed 100% discrimination by both markers while matK showed 63% success in discriminating species within a genus compared to rbcL (50%). A total of 22 novel barcodes which were found effective in discriminating and identifying the evolutionary links among the species under study were finally developed and submitted to the Barcode of Life Database (BOLD).
To Cite this article:
Irfan, S., S. Shabbir A. Rana, M. Mushtaq, I.A. Sani, R. Kanwal, S. Ullah, D. Shahid, R. Jabeen and N. Ahmed. 2024. Barcoding of Asteraceae plants of juniper ecosystem Ziarat, Balochistan. Pak. J. Bot., 56(5): DOI: http://dx.doi.org/10.30848/PJB2024-5(33)
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