PJB-2023-100
Effects of Cercospora sojina Hara stress on DNA methylation in soybean
Jia-Wen Sun, Li-Yuan Liu, Jia-Ning Cui, Jia-Yue Zhai, Hai-Ling Zhang and Quan-Wei Wang
Abstract
Epigenetic mechanisms significantly influence plant stress responses, yet their role in soybean (Glycine max) responses to stress triggered by Cercospora sojina Hara infection remains unexplored. In this study, soybean strains Longpin 09-85 and Yulong 09-521, with respective high susceptibility and high resistance to C. sojina Hara infection, were analysed for differences in infection-induced DNA methylation and changes in gene expression using the methylation-sensitive amplified polymorphism (MSAP) technique. Post-infection, both strains exhibited increased genomic DNA demethylation that was significantly more pronounced in the Longpin 09-85 strain, thereby reducing the Longpin 09-85:Yulong 09-521 DNA methylation ratio. Furthermore, the difference between stress-induced methylation and demethylation changes in DNA of the susceptible strain was significantly greater than that of the resistant strain, suggesting an important role for genomic DNA methylation in soybean resistance to stress induced by C. sojina Hara infection. Analysis of the total of 22 MSAP products that were differentially methylated between strains revealed four products that encoded proteins with homologies to proteins with known functions, as well as diverse types of methylation changes occurring in response to C. sojina Hara infection-induced stress. These findings establish a foundation on which to build future investigations of molecular mechanisms governing soybean responses to C. sojina Hara-induced stress
To Cite this article:
Sun, J.W., L.Y. Liu, J.N. Cui, J.Y. Zhai, H.L. Zhang and Q.W. Wang. 2024. Effects of Cercospora sojina Hara stress on DNA methylation in soybean. Pak. J. Bot., 56(5): DOI: http://dx.doi.org/10.30848/PJB2024-5(14)
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