PJB-2023-172
Hairy root cultures as a potential tool for the biosynthesis of active compounds in Catharanthus roseus
Aisha Mohamed Abdelkawy, Shifaa O. Alshammari, Hebat-Allah A. Hussein, Sahar K.M. Kenawy, Inas M.M. Abou El-Enain, Eman S. Abdelkhalek, Abeer S. Meganid and Ahmed A. Elateeq
Abstract
Catharanthus roseus (L.) G. Don is a medicinal plant that produces terpenoid indole alkaloids (TIAs) of immense value. Vinblastine (VBL) and vincristine (VCR) are both anticancer drugs. Hairy root cultures (HRCs) originated from leaf, stem, and root explants of C. roseus using Agrobacterium rhizogenes. The binary plasmid pBI121-containing A. rhizogenes strains (A4 and 15834) was utilized. A4-infected leaf explants converted hairy roots more effectively than strain 15834 after 60 days. In 90-day HRCs, the frequency of VCR and VBL was higher. After 60 days of cultivation, A4 strain-converted leaf-derived HRC enhances VCR and VBL content, as well as fresh or dry biomass weight. The PCR fingerprints were confirmed. Our findings showed that HRCs might be used as a promising technique for hairy root biomass and biotechnological synthesis of VBL and VCR from C. roseus medicinal plants
To Cite this article:
Abdelkawy, A.M., S.O. Alshammari, H.A.A. Hussein, S.K.M. Kenawy, I.M.M. Abou El-Enain, E.S. Abdelkhalek, A.S. Meganid and A.A. Elateeq. 2024. Hairy root cultures as a potential tool for the biosynthesis of active compounds in Catharanthus roseus. Pak. J. Bot., 56(4): DOI: http://dx.doi.org/10.30848/PJB2024-4(29)
Download
