PJB-1994-47
Identification of different wheat genotypes through polymorphism based on random amplified polymorphic DNA (RAPD)
S. Farooq, T.M. Shah, E. Askari, A.A. Zaidi, M. Arif and N. Iqbal
Abstract
Polymerase chain reaction was used to detect polymorphism in 10 different salt-tolerant wheat introgression lines produced through wide hybridization, 2 cultivated hexaploid wheat varieties, a tetraploid wheat variety and salt-tolerant accession D of Aegilops cylindrica. DNAs extracted from fresh leaves by the CTAB method were amplified using 25 µ.L reaction volume in a Perkin Elmer Thermal Cycler and randomly sequenced 10 mer synthetic primers. Based on amplification reactions, all the primers were divided into 4 categories. Category A primers reacted with all the wheat lines, category B with none, category C with 10-14, and category D with 3-7 wheat lines. Of the 18 reactive primers, 11(61%) detected polymorphism in all the test material. The level of polymorphism was low and ranged from 1.2 to 22.8%. Most of the primers produced monomorphic bands including very intense, easily visible and less visible bands. Of the 9 salt-tolerant introgression wheat lines, 6 were identified on the basis of polymorphic bands. Our study indicated possibilities for using Randon Amplified Polymorphic , DNA (RAPD) markers to detect specific variations in the genome that could be used for varietal finger printing
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