PJB-1994-48
Morphogenesis in the suspension culture of Nicotiana tabacum cv. varginica: The effect of kinetin
Khalida Khatoon
Abstract
Suspension cultures of Nicotiana tabacum cv. Virginica established from epidermal tissue-derived callus on medium supplemented with 5x10-6M NAA alone or with 5xl0-7 M kinetin when subcultured on auxin licking morphogenetic medium containing kinetin, exhibited morphogenesis. The mode of regeneration was dependent on the concentration of kinetin. Low concentrations of kinetin favoured root. and embryoIe structure development while high concentration of kinetin induced tightly aggregated clusters of shoots. The regenerated structures showed a general tendency towards a gradual increase in the number of shoots with a concomitant decrease in their size at increasing concentrations of kinetin. Histological study of the embryo-like structures revealed their resemblance with the zygotic embryos.
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