PJB-2023-361
Optimization of fermentation medium and fermentation conditions of biocontrol strain L131 against Alternaria alternata
Xinyue Ma, Xilin Sun, Xi Zhao, Yufan Wang, Lingyue Zhu, Wenxing Li and Xiaomei Wang
Abstract
Early blight is one of the most widespread and devastating diseases of tomato. Our previous research has isolated and screened a biocontrol strain L131 in vegetable rhizosphere soil, which has good inhibitory effect against Alternaria alternata and was identified as Penicillium citrinum. The fermentation medium and fermentation conditions were optimized by single-factor experiments, orthogonal experiments and response surface experiments. The results showed that the optimal fermentation medium was lactose 27.33 g·L-1, beef paste 2.39 g·L-1 and NaCl 1.70 g·L-1. A model was developed using Design Expert software and the predicted inhibition rate was 81.60% under the above conditions. The actual inhibition rate obtained after validation was 81.55%, which was close to the predicted value. It was 15.14% higher than that before optimization. Meanwhile, the optimal fermentation conditions were as follows: liquid volume 100/250 mL, 20℃, and 7 d. This experiment provides the basis for the development of large-scale fermentation and biopesticides in future production
To Cite this article:
Ma, X., X. Sun, X. Zhao, Y. Wang, L. Zhu, W. Li and X. Wang. 2024. Optimization of fermentation medium and fermentation conditions of biocontrol strain L131 against Alternaria alternata. Pak. J. Bot., 56(4): DOI: http://dx.doi.org/10.30848/PJB2024-4(38)
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