PJB-2024-1527
Genetic relationships of Asparagus species (Asparagaceae) based on biochemical and molecular markers
Zhiyong Zhang, Muhammad Idrees, Muhammad Irshad, Fang Wang, Wennian Chen, Nan Zhang, Muhammad Arfan, Xu Zeng, Yongqing Jiao, Shazia Sultana and Rehan Naeem
Abstract
The genetic relationships of Asparagus species (Asparagaceae) from Pakistan were evaluated using sodium dodecyl sulphate poly-acrylamide gel electrophoresis (SDS-PAGE) and PCR-based random decamer markers. Protein profiling of seed proteins of Asparagus species yielded 19 bands with 63.2% polymorphism. The indices of similarity ranged from 0.61 to 0.97, with an average of 0.55. Asparagus species were divided into six clusters based on cluster analysis, Cluster I consists of A. officinalis and A. officinalis cv. Taranga, cv. Gersengum, and cv. Para selection. Cluster II consists of A. officinalis cv. Appolo, cv. Abril and cv. Huchel. Cluster III consists of A. capitatus and A. gracilis. Cluster IV, V and VI contain A. adscendens, A. racemosus and A. densiflorus, which have formed distinct clades. RAPD markers using eight primers produced a total of 50 fragments, of which 36 (72%) were polymorphic and 14 (28%) were monomorphic bands. The Asparagus species were divided into four major clusters based on neighbor joining method of cluster analysis. Cluster I consists of Asparagus officinalis, cluster II comprise of Asparagus officinalis cultivars (Abril, Apollo, Gersengum, Huchel, Para and Taranga). Cluster III consists of six Asparagus wild species (A. adscendens, A. densiflorus, A. racemosus, A. setaceus, A. plumosus and A. capitatus), whereas Cluster IV contain only Asparagus gracilis (synonym of A. asiaticus), which was formed a separate clade from the rest of the species. It was noticed that PCR-based RAPD markers are more effective at resolving species relationships than allelic patterns of seed storage proteins
To Cite this article:
Zhang, Z., M. Idrees, M. Irshad, F. Wang, W. Chen, N. Zhang, M. Arfan, X. Zeng, Y. Jiao, S. Sultana and R. Naeem. 2024. Genetic relationships of Asparagus species (Asparagaceae) based on biochemical and molecular markers. Pak. J. Bot., 56(3): DOI: http://dx.doi.org/10.30848/PJB2024-3(26)
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