PJB-2007-102
KINETICS OF INVERTASE PRODUCTION BY SACCHAROMYCES CEREVISIAE IN BATCH CULTURE
IKRAM-UL-HAQ AND SIKANDER ALI
Abstract
The present study is concerned with the improved invertase production by Saccharomyces cerevisiae on kinetic basis. The wild-culture (IIB-1) and putative mutant (NA6) of S. cerevisiae were compared on time course basis when grown in batch culture. The values for Yx/s, Yp/s and Yp/x with urea were significantly improved (p≤0.05) over the control (peptone+yeast extract) as well as peptone supplementation. The maximum growth in terms of volumetric rate for cell mass formation (Qx), 48 h after the incubation was only marginally different from control. The values of both Km and Vmax of the invertase from mutant culture were significantly improved, as were the specificity constant and kcat for the enzyme. The activation energy and substrate binding for sucrose fermenting were found to be highly significant for yeast culturing.
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