PJB-2007-104
NODULATION OF SESBANIA SPP., BY INTRODUCED RHIZOBIA IN COMPETITION WITH NATURALIZED STRAINS IN DIFFERENT SOIL TYPES
FAUZIA Y. HAFEEZ*, **FARRUKH NAEEM**, NAZIA SHAHEEN AND KAUSAR A. MALIK
Abstract
Seven rhizobial strains were isolated from Sesbania aculeata and three from Sesbania grandifolia from three different soil types. These strains were characterized for their morphology and carbon source utilization. The affectivity of these strains was confirmed by re inoculating the host and were effective on both Sesbania spp. The strains Sa1 and Sa2, however found to be more effective having nitrogenase activity ranging from 3.7-85.2 and from 4.8-77.7 m moles of C2H4 produced h-1 g-1 nodule dry weight respectively. The consortium of Sa1, Sa2, Sg1 and Sg2 was also found to be highly effective in both Sesbania spp. In competition with the indigenous population, the strains Sa1 and Sa2 were found to be most competitive among the others. Fluorescent antibody studies showed the nodule occupancy ranged from 30-100% by Sa1 and Sa2 in both species. Response of inoculum was better in soils 1 & 2 which were low in nutrient level than in soil 3.
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