PJB-2007-140
IDENTIFYING GENETIC VARIATION IN GOSSYPIUM BASED ONSINGLE NUCLEOTIDE POLYMORPHISM
SAGHIR AHMAD1, TIANZHEN ZHANG3, NOOR-UL-ISLAM4, TAYYABA SHAHEEN5 AND MEHBOOB-UR-RAHMAN5
Abstract
Various molecular markers such as RAPD, RFLP, AFLP, SSR and EST-SSR are being employed to detect genetic diversity and for marker-assisted selection (MAS) in cotton. But little information is available regarding the use of Single Nucleotide Polymorphisms (SNPs) in this crop. In the present study, polymorphism in the FIF1 gene, important in regulating cotton fiber development in Gossypium barbadense L., was studied among G. hirsutum cv. TM-1 and G. barbadense cv. Hai7124. FIF1 genes from G. hirsutum cv. TM-1 and G. barbadense cv. Hai7124 were cloned on the basis of its reported sequence in G. arboreum L. Three bases substitutions were detected at the 188-
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