PJB-2007-141
IN VITRO REGENERATION OF BRASSICA NAPUS L., CULTIVARS (STAR, CYCLONE AND WESTAR) FROM HYPOCOTYLS AND COTYLEDONARY LEAVES
HAZRAT ALI1, ZAHIR ALI2, HAIDAR ALI3, SULTAN MEHMOOD2 AND WIQAR ALI2
Abstract
Regeneration protocols were established for Brassica napus L., cultivars viz., Star, Cyclon and Westar. The conditions were optimized for callus induction, shoot and root regeneration. Seeds were surface sterilized using 70% alcohol, 0.01% HgCl2 and few drops of Tween20 for ten minutes. Which reduced seed borne contamination. Callus induction ability was evaluated by using different concentration of 2, 4-D in combination with 0.5mg/L BAP and 0.5mg/L Silver nitrate, which was used for the first time in MS medium and efficient callus was produced using 0.5mg/L 2, 4-D. Silver nitrate in callus induction media resulted in green callus. Shoots were regenerated on callus using different concentrations of NAA with 2mg/L BAP and 5mg/L Silver nitrate in MS medium. 67-82% shoots were regenerated on media having 0.1mg/L NAA. The shoots were then rooted and best results were obtained on media having 0.3mg/L IBA in half strength MS medium.
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