PJB-2007-144
COMPARISON OF SUCROSE AND SORBITOL AS MAIN CARBON ENERGY SOURCES IN MICROPROPAGATION OF PEACH ROOTSTOCK GF-677
TOUQEER AHMAD, NADEEM AKHTAR ABBASI, ISHFAQ AHMAD HAFIZ AND ANSAR ALI
Abstract
The influence of two carbon sources, sorbitol and sucrose on In vitro shoot proliferation and rooting of peach rootstock GF 677 was compared at 15, 30, 45 and 60 g l-1 concentrations. The highest number of shoots per proliferated explant, usable shoots and shoots having maximum fresh weight were obtained on sorbitol at 30 g l-1. GF 677 does not seem to be an efficient utilizer of sucrose for In vitro shoot proliferation. Similarly best root development in terms of rooting percentage, number of roots per rooted explant and roots > 1.5 cm in length was also found on sorbitol at 30 g l-1. Sorbitol is therefore better carbon source than sucrose for the development of In vitro shoot proliferation and rooting of peach rootstock GF 677.
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