PJB-2007-197
FFECT OF PERMANENT AND TEMPORARY IMMERSION SYSTEMS ON BANANA MICRO-PROPAGATION
IKRAM-UL-HAQ* AND MUHAMMAD UMAR DAHOT
Abstract
For the establishment of a micro-propagation protocol for banana (Musa spp.) cv Basrai, meristematic stem cuttings were used as an explant. A number of cultures were maintained on MS medium supplemented with various auxins and cytokinins, of which a combination of IAA and BA for organogenesis and BA only for shoot induction/multiplication were considered as good agents for In-vitro propagation of banana. Micro-propagation efficiency was significantly (P >0.005) increased, when organogenesis was carried out by culturing on MS medium supplemented with 10.0 mM BA; 15.0 mM IAA and solidified with 3.60 g/L phytagel for 3-weeks, while shoot induction (1.0 g/L phytagel) and its multiplication (2.0 g/L phytagel) on MS medium supplemented with 10.0 mM BA for 2 and 3-weeks respectively. 17.65±0.50 plantlets per micro-stem cutting were developed through this protocol. Among others, in one medium (6.0 mM TDZ and 4.0 mM NAA or/and 10.0 mM BA) callus formation was observed but later on cultures proceeded to death, instead of multiplication. The phenolic oxidation was inhibited through the addition of L-cystein (30.0 mg/L) in each culture. Roots developed within 2-weeks, by culturing on MS basal medium supplemented with IBA (0.1 mg/L). Through this protocol, complete and normal micro-propagated plantlets were obtained within 2-3 months.
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