PJB-2007-216
RAPID CLONAL PROPAGATION OF CHRYSANTHEMUM THROUGH EMBROYOGENIC CALLUS FORMATION
IHSAN ILAHI, MUSARRAT JABEEN AND S. NAZNEEN SADAF
Abstract
Chrysanthemum nodal explants were cultured on ½ X MS medium containing different combinations of growth hormones. A reasonable callus formed on explants when MS contained 0.5 mg. cm-3 each of BAP and NAA after about 3 weeks. Similar results were obtained on MS + 0.5 mg. cm-3 BAP + 0.1 mg. cm-3 NAA or 1.0 mg. cm-3 BAP + 0.1 mg. cm-3 NAA. A maroonish embryogenic callus was formed on MS containing BAP only. This callus, then exhibited various embryonic developmental stages and gave rise to normal seedlings. These were acclimatized and transferred to natural conditions. The plants ultimately flowered exhibiting superior quality and early maturity compared to stock plants.
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