PJB-2007-219
IN VITRO INDUCED MUTATION FOR SCREENING OF RED ROT (COLLETOTRICHUM FALCATUM) RESISTANCE IN SUGARCANE (SACCHARUM OFFICINARUM)
AAMIR ALI1*, SHAGUFTA NAZ2, S. SARWAR ALAM3 AND JAVED IQBAL4
Abstract
Explants from leaf, shoot apical meristem and parenchymatous pith were cultured on MS medium supplemented with different concentrations of 2,4-D either alone or in combination with BAP. MS medium containing 3.0 mg/l 2,4-D exhibited maximum callus induction. Seven week old well developed calli were either treated with different concentrations of sodium azide ranging from 1.0 to 5.0 mg/l or irradiated with 10 Gy
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