PJB-1995-16
UTILIZATION OF RAPD MARKERS FOR THE IDENTIFICATION OF CULTIVATED AND WILD RICE SPECIES
S. FAROOQ, T. M. SHAH, M. ARIF AND N. IQBAL
Abstract
Random Amplified Polymorphic DNA (RAPD) markers were generated in Perkin Elmer DNA Thermal Cycler using genomic DNAs extracted from different rice varieties/species and 6 synthetic 10 mer primers of series -S. Material included in the study comprised 5 cultivated and 11 wild salt tolerant and sensitive varieties/spccies with different genomes and ploidy level. The scorable DNA fragments amplified by different primers ranged between 22 and 47 fragments per primer. Of the total 212 fragments that were amplified, 99 fragments were scored as potential genetic markers to be used for cultivar identification. Of the 99 markers, 50 (51 %) were cultivar specific while 49 markers were genome specific. One marker appeared only in salt tolerant cultivated rice varietie8 while another appeared both in wild and cultivated but salt tolerant varieties. Based on these markers, 3 out of 5 cultivated and 5 out of 11wild rice species were distinguished from cach other. Inter and intraspecific variations were also detected. The markers identified in the present study can bc used to check seed quality, for fingerprinting of a specific cultivar and to detect transfer of genetic material from wild species to cultivated varieties. The results showed the potential of RAPD markers for gene lagging experiments particularly tagging genes for salt tolerance.
To Cite this article:
Download
