PJB-2008-34
CALLUS FORMATION AND PLANTLETS REGENERATION FROM HYPOCOTYL OF BRASSICA NAPUS BY USING DIFFERENT MEDIA COMBINATIONS
MUHAMMAD MUNIR, HAMID RASHID*, M. RAUF, ZUBEDA CHAUDHRY* AND M. SHAHJAHAN BUKHARI
Abstract
The genus Brassica includes several important crop species. Canola (Brassica napus L.) is considered as the most important source of vegetable oil and protein rich meal worldwide. Effect of 5 different callusing media (CIM1 to CIM5) and regeneration media (RM1 to RM10) supplemented with different concentrations of hormones were studied for callus induction and regeneration of four cultivars of Brassica napusc viz., Dunkeld, Oscar, H-19 and Rainbow. The experimental work was carried out at the Agricultural Biotechnology Program, National Agricultural Research Centre, Islamabad during March 2004 to January 2005. The results indicated that among the 5 media combinations, MS media supplemented with 0.5mg /l IAA
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