PJB-2008-68
INDUCED GENETIC VARIABILITY IN CHICKPEA (CICER ARIETINUM L.) II. COMPARATIVE MUTAGENIC EFFECTIVENESS AND EFFICIENCY OF PHYSICAL AND CHEMICAL MUTAGENS
TARIQ MAHMUD SHAH*, JAVED IQBAL MIRZA1, MUHAMMAD AHSANUL HAQ AND BABAR MANZOOR ATTA
Abstract
Comparative mutagenic effectiveness and efficiency of gamma rays and Ethyl methane sulphonate (EMS) were studied in two desi (Pb2000 and C44), one kabuli (Pb1) and one desi x kabuli introgression line (CH40/91) of chickpea. The treatments included two doses each of gamma rays and EMS calculated on the basis of their LD30. The results revealed that EMS was almost seven times more effective and its efficiency was two times higher than that of gamma rays. Mutagenic effectiveness and efficiency were found to depend upon mutagen type and the genotype and both were higher at lower doses of EMS in three genotypes except in desi genotype C44. The overall trend of mutagenic effectiveness and efficiency in both gamma radiation and EMS was in the order i.e. CH40/91>Pb2000>Pb1>C44. The introgression line desi x kabuli genotype was found to be most resistant towards mutagenic treatments than desi and kabuli types.
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