PJB-1995-31
SOME BIOLOGICAL PHYSICAL AND SEROLOGICAL PROPERTIES OF POTATO LEAF ROLL VIRUS (PLRV) IN PAKISTAN
M. ARIF, S.M. MUGHAL, S. KHALID AND S. HASSAN*
Abstract
Mean incidence of potato leaf roll virus (PLRV) in the plains of Pakistan was recorded as 44% in general, 53 % in the market or uncertified seed and 11% in certified or improved seed. The virus was successfully transmitted by side cleft grafting of infected potato to Datura slramonium and Physalis jloridana which proved useful hosts for the maintenance and propagation of the virus. The virus was purified through extraction in phosphate or citrate buffer and precipitation with polyethylene glycol followed by 2-3 cycles of differential centrifugation. The average yield of virus was 0.265 mg per kg of source tissue. The virus sedimented as single component in sucrose density gradients, reacted positively against potato leaf roll virus (PLRV) antiserum in gel immunodiffusion tests and absorbed UV light with a 260/280 ratio of I. 7. Isometric particles of 25 nm in diameter were observed under electron microscope (EM). The virus identity was also confirmed by Enzyme Linked Immunosorbent Assay (ELISA) and serological specific electron microscopy (SSEM).
To Cite this article:
Download
