PJB-2008-282
PHENOLIC CONTENT IN VITRO CULTURES 0F CHICK PEA (CICER ARIETINUM L.) DURING CALLOGENESIS AND ORGANOGENESIS
SHAGUFTA NAZ1, AAMIR ALI2 AND JAVED IQBAL3
Abstract
The callogeneic response of different explants i.e. cotyledon, leaf, node, shoot and root apices of Cicer arietinum was investigated on MS basal media supplemented with different concentrations of auxins and cytokinins. A combination of BAP and 2,4-D or NAA enhanced proliferation response. Of different combinations MS+1.0 2,4-D+0.5 NAA+0.5 BAP mg/l proved exceptionally good both for callus induction and proliferation. Callus masses initiated from different explants were maintained from main to 10thsub cultures. The morphogenic behaviour of different calli was also investigated. It was observed that the rate of browning and necrosis was increased with the increasing subcultures. To elucidate the biochemical basis of growth inhibition in non-morphogenic and morphogenic calli, total phenolics were estimated from main to 10th sub cultures both quantitatively and qualitatively. A gradual increase in total phenolic content was noticed from main to 10th sub cultures in both non-morphogenic and morphogenic calli. Non-morphogenic calli exhibited higher phenolic content as compared to morphogenic calli. The qualitative analysis of phenolic compound was also in conformity with quantitative data as the number of phenolic spots increased from main to 10th subcultures.
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