PJB-1995-47
CONGRUENCE BETWEEN MORPHOLOGICAL AND MOLECU LAR PHYWGENETIES IN PROSARTES (LILIACEAE)
ZABTA KHAN SHINWARI
Abstract
cp DNA variations were examined in 5 genera of the tribe Polygonatae, for 60 probe-enzyme combinations, to study Disporum-Prosartes generic separation problem. The shared fragments were counted, index of similarity and distance matrix was calculated. The distance within the genus Prosartes was 0.033, while it differed from Disporum in 0.34-0.342. The difference of Prosartes from Streplopus was 0.217, while between Disporum and Uvularia was 0.164-0.171. The data was examined using Neighbour Joining (NJ) and Fitch-Margoliash methods. The divergence between Disporum and Prosartes was greater than that between Disporum and Uvularia or Prosartes and Streplopus. Within the genus Prosartes, P. maculala was different from the rest of the Prosartes species. rbcL gene sequence data revealed that north American Prosartes and Asian Disporum species differed by 90-96 substitution (100d= 6.92-7.41). The base substitution between Disporum and Prosartes is much greater than that between Prosartes and Streplopus (14-16, 100d= 1.03-1.18) or that between Disporum and Uvularia (23-26, 100d= 1.70-2.01). The results support recent morphological, karyological and molecular arguments of the restoration of genus Prosartes as an independent genus. The results also indicate that Uvularia is closly related to Dispomm while Streplopus is closer to the genus Prosartes.
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