PJB-2009-17
EFFECT OF GA3 ON REGENERATION RESPONSE OF THREE TOMATO CULTIVARS (LYCOPERSICON ESCULENTUM)
AMBER AFROZ1, ZUBEDA CHAUDHRY2, RASHEED KHAN1, HAMID RASHID3* AND SABAZ ALI KHAN1
Abstract
This study was conducted for developing a high frequency regeneration system in short time span using GA3, as a pre-requisite for the genetic transformation in tomato cultivars. Effects of GA3 were investigated on regeneration efficiencies and days to maturity of three varieties of tomato Lycopersicon esculentum (using hypocotyls and leaf discs as explant source). 0.5 mg/l Indole acetic acid (IAA) and 0.5-2.5 mg/l of benzyl amino purine (BAP) were used alone or in combination with GA3 2mg/l on MS media. Regeneration was significantly higher with different treatments used in combination with GA3. It was increased from 57.33% to 70% in Avinash, followed by Pusa Ruby 51.66% to 67.22% and from 53.2% to 60% in case of Pant Bahr when hypocotyls were used as explant source. Same trend was followed in case of leaf disc derived regeneration, although it was less pronounced. Regeneration was increased from 68% to 73% in Avinash followed by Pusa Ruby 68.5% to 72.33 %. Inclusion of GA3 in the media also significantly reduced the days to regeneration (20-25) as against 40-45 days when GA3 was excluded from media in all three varieties of tomato cultivars.
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