PJB-2009-44
AGROBACTERIUM MEDIATED TOBACCO TRANSFORMATION WITH RICE FAE GENE AND SEGREGATION ANALYSIS OF T1 GENERATION
KHIZAR HAYAT BHATTI1,2* AND CHAOZU HE1
Abstract
The transformation of plants through Agrobacterium is very important to impart desirable traits. Usually plants are subjected to various abiotic and biotic stresses, which affect the growth and metabolism. Plants evolve certain mechanisms to cope with the prevailing stressful conditions. Among them, cuticular waxy coating layer may serve as protective barrier to deter water loss as well as pathogens assault. The rice OsFAE encodes a protein involving elongation of fatty acids to form very long chain fatty acids (VLCFAs), necessary for cuticular wax biosynthesis. The OsFAE gene in the sense sequence was placed under the control CaMV35S promoter. The Agrobacterium mediated transgenic tobacco plants were obtained using tissue culture techniques. The PCR analysis of T2 generation confirmed the incorporation of transgene into the tobacco genome. The selection of transgenic plants was made on hygromycin. Segregation analysis of T1 generation revealed that most of the transgenic lines showed typical 3:1 Mendel’s segregation ratio.
To Cite this article:
Download
