PJB-2009-78
DEVELOPMENT OF RAPD-BASED MOLECULAR MARKERS FOR CHROMOSOME 5A OF COMMON WHEAT
AMJAD ALI, IMTIAZ AHMAD KHAN, HABIB AHMAD1* AND SAJID UL GHAFOOR
Abstract
During the present study, 6 deletion lines (del 5AS-3, del5AS-7, del5AS-10, del5AL-10, del5AL-12 and del5AL-23) of chromosomes 5A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers specific for short and/or long arm of chromosome 5A and one deletion line (5DS-1) used as a positive control. Out of the 7 RAPD primers used, OPA-07 showed useful polymorphism for long arm deletion line del5AL-23. By comparing the C-banding karyotype of the deletion lines, it is inferred that the primer (OPA-07) anneals to the distal half of the long arm of chromosome 5A.
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