PJB-2009-93
A FACILE PROCEDURE FOR EFFICIENT PLANTLET REGENERATION AND FRUITING WITHOUT VERNALIZATION IN THELLUNGIELLA SALSUGINEA
WEIYANG SUN1,2, JIANFANG LI2, YANXIU ZHAO1 AND HUI ZHANG1*
Abstract
A facile and rapid system for efficient shoot regeneration and callus keeping was developed for Thellungiella salsuginea (Pall.) O. E. Schulz. Three kinds of explants including basal leaves, stems and anthers of vernalized plants were adopted here. Calli were formed from all these explants on MS medium supplemented with various plant hormone treatments. The highest callus inducing ratio for anther explants were observed on MS medium supplemented with 2.0 mg/l 2,4-D and 1.0 mg/l ZT zeatin, while for both stem and leaf explants 1.0 mg/l 2,4-D
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