PJB-2009-192
FOURIER TRANSFORM INFRARED SPECTOMETRY STUDY ON EARLY STAGE OF CADMIUM STRESS IN CLOVER LEAVES
Z.L.WEI 1*, L. DONG 2 AND Z. H. TIAN 1
Abstract
A technique based on Fourier transform infrared spectrometry (FTIR) was developed to detect the changes of chemical composition in the cadmium-stressed clover leaves. The obtained IR spectra were further processed by de-convolution and curve fitting for quantitatively examine the chemical contents and structure changing. Within 1h of 15mg/L Cd stress, the changes of structure and content of compounds, such as proteins, lipids and cell wall pectin synthesis, was more remarkable in clover leaves. After 24 hours of Cd stress, except saccharide, all the compounds were restored to near the control level. Our experiment suggested that the FTIR technique is applicable on study of plant stress responding.
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