PJB-2009-223
SPLIT ROLE OF PLASMID GENES IN THE DEGRADATION OF CHLORPYRIFOS BY INDIGENOUSLY ISOLATED PSEUDOMONAS PUTIDA MAS-1
MUNAZZA AJAZ1, NUSRAT JABEEN2, TASNEEM ADAM ALI2 AND SHEIKH AJAZ RASOOL2*
Abstract
A chlorpyrifos degrading bacterium Pseudomonas putida MAS-1 was isolated from the cotton grown soil of NIAB, Faisalabad, Pakistan. Genetic studies based on plasmid curing and electroporation mediated transformation were performed on this bacterium. The bacterium lost the property to grow on the nutrient agar containing 10mg/mL chlorpyrifos after acridine orange mediated curing. The plasmid (bearing chlorpyrifos degrading determinants / genes) was isolated and transferred into E. coli DH5a. The transformants however, could not resist and grow in the chlorpyrifos containing medium. It may be concluded that chlorpyrifos degradation Pseudomonas putida MAS-1 is accomplished by the combined action of plasmid and chromosomal genes.
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