PJB-2009-244
GENETIC DIVERSITY IN ANNUAL WILD SOYBEAN (GLYCINE SOJA SIEB. ET ZUCC.) AND CULTIVATED SOYBEAN (G. MAX. MERR.) FROM DIFFERENT LATITUDES IN CHINA
WEI AN1, HONGKUN ZHAO2, YINGSHANDONG1,2*, YUMIN WANG2, QIYUN LI2 , BINGCHANG ZHUANG2, LEI GONG1 AND BAO LIU1*
Abstract
A total of 50 accessions of annual wild soybean (Glycine soja) and cultivated soybean (G. max) were used to study genetic variations by three genetic fingerprinting systems, RAPD, SSR, and AFLP. Ninety polymorphic RAPD bands were generated by 10 decamer primers, 56 SSR loci were obtained by 12 pairs of primers and 935 bands were resolved by 11 AFLP primer combinations. Based on the marker data, an UPGMA-cluster analysis separated the soybean collections into two groups that corresponded to wild soybean and cultivated soybean respectively. Genetic diversity calculation suggested that the diversity of G. soja was higher than that of G. max. Specific informative bands for G. soja and G. max were detected, which provided molecular evidence in support of the clear distinction of G. soja and G. max. Finally, it was found that environmental factors may play important roles in soybean evolution. A comparison of the three molecular markers suggests that AFLP and SSR are more suitable than RAPD for genetic diversity studies in soybean.
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