PJB-2010-87
MULTIPLE SHOOT REGENERATION IN CLERODENDRUM INCISUM L., – AN ORNAMENTAL WOODY SHRUB
SONU GOYAL*1, ANWAR SHAHZAD2, MUHAMMAD ANIS2 AND SAMIULLAH KHAN1
Abstract
A rapid and efficient protocol for induction of multiple shoots from nodal explants of an ornamental woody shrub, Clerodendrum incisum L., var. macrosiphon was developed. Nodal explants were inoculated on MS medium containing different concentrations of 6-Benzyladenine (BA) or Kinetin (Kn) alone. Murashige & Skoog (MS) medium supplemented with BA (5 µM) induced maximum number of shoots. The shoots were rooted on half strength of MS medium supplemented with α-naphthalene acetic acid (NAA) and rooted plantlets were established in soil as phenotypically normal mature plants.
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