Paper Details

PJB-2010-126

AN EFFICIENT METHOD OF PROTOPLAST ISOLATION IN BANANA (MUSA SPP.)

ABDULLAH KHATRI1, MUHAMMAD UMAR DAHOT2, IMTIAZ AHMED KHAN1 AND GHULAM SHAH NIZAMANI1
Abstract

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Protoplast cultures are essential commodity for transformation and this can be achieved through electroporation and somatic hybridization. Like other monocot systems, embryogenic cell suspension is the material of choice for the isolation of protoplasts. In banana protoplasts have been successfully isolated but so far sufficient quantities of protoplasts for practical application are not routinely met. Enzyme mixture comprises of 3% cellulose R-10

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