PJB-2010-158
IN VITRO CONSERVATION OF CADABA HETEROTRICHA STOCKS, AN ENDANGERED SPECIES IN PAKISTAN
HAIDER ABBAS1* AND MUHAMMAD QAISER2
Abstract
In vitro conservation protocol was developed for Cadaba heterotricha, an endangered species reported from Southern Pakistan. This species has been subjected to various threats including habitat loss and over-exploitation, need an urgent conservation measures. Seeds collected from the wild, were used as initiating material. While, nodal segments of in vitro germinated seedlings were employed to induce multiple shoot regeneration. Full strength MS medium supplemented with 2.5 mg/l BAP and 0.5 mg/l Kin along with 0.5 mg/l NAA helps in attaining the highest number (16.0) of shoots with 100% shoot regeneration frequency. The cultures maintained in good conditions with subculture after 6-7 months. IAA at the level of 3.0 mg/l gave maximum number (2.70) of rooting along with the highest rooting frequency (80%).
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