PJB-2010-183
CHARACTERIZATION OF CASTOR BEAN GENOTYPES UNDER VARIOUS ENVIRONMENTS USING SDS-PAGE OF TOTAL SEED STORAGE PROTEINS
NASIR MAHMOOD CHEEMA1*, MUHAMMAD AZIM MALIK2, GHULAM QADIR2 AND MALIK ASHIQ RABBANI3
Abstract
Castor bean (Ricinus communis L.) is an indeterminate, non-edible oil seed crop grown in low rainfall regions of semi-arid tropics and sub-tropics. The present work was conducted to see the feasibility of electrophoresis for intra-specific characterization of castor bean on the basis of their total seed storage proteins. The protein profiles of all the four accessions were very much alike, both in number of bands and in their distribution on the gel, even though accessions represented a wide range of geographic origin. Occasionally, variation was observed in the density or sharpness of bands. The seed protein patterns were also uniform among various accessions grown under various environments. On the basis of banding pattern, polypeptides could be divided into three regions, A to C, equivalent to increasing Rf value and decreasing molecular weight. The present investigation revealed no variation in different accessions and under different environments with regards to their total seed protein profiles. The results clearly showed that it was impossible to discriminate various genotypes from each other, as they were characterized by same banding patterns. However, it might be useful to distinguish diverse forms of it from one another. The present investigation revealed very limited variation in castor bean genotypes in Pakistan.
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