PJB-2010-212
DIVERSITY OF GLUTELIN ΑLPHA SUBUNITS IN RICE VARIETIES FROM PAKISTAN
NADAR KHAN1*, NAUSHAD ALI2, MALIK ASHIQ RABBANI1, MUHAMMAD SHAHID MASOOD1
Abstract
The diversity of glutelin α subunits in thirty-two rice varieties from Pakistan was assessed using higher temperature sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Five variation types i.e. Type-I, Type-II, Type-III, Type-IV and Type-V on the basis of variation in molecular size and intensities of glutelin α subunits were identified. Six rice varieties, i.e., Khushboo-95, Shahkar, Sonahri-Sugdasi, Sugdasi-Bengalo, Sugdasi-Ratria and Sugdasi-Sadagulab exhibited Type-IV variation having comparatively higher glutelin content. These varieties may be important genetic resources for improving glutelin content of rice seed. Rice variety Jhona-349 exhibited Type-V variation type and displayed unique variation by showing the presence of unique bands. Accordingly, this variety might have some unique glutelin genes and need to be further explored for nutritional quality improvement of rice seed.
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