PJB-2010-225
EFFECT OF A STRONG ENZYME DENATURANT (UREA) ON THE STABILITY OF SOLUBLE ACID INVERTASES FROM SUGARCANE
ALTAF HUSSAIN1, ZAFAR IQBAL KHAN2*, KAFEEL AHMAD2, MUHAMMAD ASHRAF1,!, EHSAN ELAHI VALEEM3* AND MUHAMMAD HAMID RASHID4
Abstract
An investigation was performed to determine the effect of urea, a strong enzyme denaturant on the stability of invertases from two cultivars of sugarcane. The stability of native soluble acid invertases (SAI) of COJ-84 (local cultivar of sugarcane) to urea showed that they were more stable than those of CP-77-400 (local cultivar of sugarcane). The stability of SAI of CP-77-400 to urea gave biphasic trend at different salinity levels, and the stability at these levels decreased significantly as compared to control. The stability of 50 mM salinity level SAI was also decreased, while at 150 mM salinity level remained unchanged. The SAI of COJ-84 secreted under saline growth media presented extreme stability to unfolding by urea. The SAI at 150 and 200 mM salinity levels gave an activation trend and did not show denaturation.
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