PJB-2010-343
MOLECULAR ANALYSIS OF GANODERMA LUCIDUM ISOLATES FROM LAHORE
GHAZALA NASIM*, MUHAMMAD ALI, AND NASIR MEHMOOD
Abstract
Isolates of Ganoderma lucidum were collected randomly from the various regions of the Lahore and Changa Manga. The samples were brought to the Lab for their DNA isolation and quantification as well. Random amplified polymorphic DNA analysis was used to determine the % variability in the genomic profiles of randomly collected Ganoderma lucidum isolates from Lahore. For RAPD analysis three primers (5’ACCTGGACAC3’, 5’GTATTGCCC3’ and 5’GCTGTAGTGT3’) were applied and designated as P1, P2 and P3 respectively. Only P1 was screened for the amplification of DNA fragments. Dendrogram of the RAPD analysis provided information of the genetic variability among the isolates.
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