PJB-2010-392
SCREENING OF MUTATED POPULATION OF SUGARCANE THROUGH RAPD
SAJIDA BIBI, IMTIAZ AHMED KHAN, ABDULLAH KHATRI, SHAFQAT YASMIN, NIGHAT SEEMA, SHAHID AFGHAN* AND M.A. ARAIN
Abstract
Variability obtained from mutation breeding (gamma rays) was examined through molecular marker techniques (RAPD). A total of 73 loci were amplified with 13 primers, out of which 75.34% were polymorphic and 24.66% were monomorphic. Fragments size ranged from 275bp-2.2kb and fragments produced by various primers ranged from 3-9 with an average of 3 fragments per primer. The highest number of loci 9 was amplified with primer B-09, while the lowest number 3 with primer B-02 and B-08. Results revealed that mutant P2(30Gy) contained three specific segment of 421bp
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