PJB-2010-439
ISOLATION, PURIFICATION, CHARACTERIZATION AND IDENTIFICATION OF VIRULENCE FACTORS OF PSEUDOMONAS SYRINGAE PV. SESAMI AND XANTHOMONAS CAMPESTRIS PV. SESAMI
SYEDA SADIQA FIRDOUS1, R. ASGHAR1*, M.I. HAQUE2, SYED NADEEM AFZAL2 , G. MURTAZA1 AND S. M. MUGHAL2
Abstract
In the present study production, characterization and identification of virulence factors produced by virulent isolates of Pseudomonas syringae pv. sesami (Psse-08/NARC 1) and Xanthomonas campestris pv. sesami (Xcs-08/NARC) were carried out. Identification was made on the basis of earlier published reference data. Previously, toxins were detected by the phytotoxic as well as growth inhibition assays. HPLC analysis of cell free culture filtrates allowed characterizing toxins activity of a single active peak obtained from virulent isolate of Psse-08/NARC 1 that produced necrotic symptoms. In the present results, the retention time of peak obtained from Psse-08/NARC 1 was same as previously reported from mangotoxin, which causes apical necrosis of mango tree. However, there was no reference data for the active peak obtained from Xcs isolates, but the single active peak showed antibacterial activity. Later characterization of active fraction from Xcs was also performed in phytotoxic assay.
To Cite this article:
Download
