PJB-2010-140
EFFECT OF DIFFERENT ANTIMICROBIAL AGENTS ON THE FIBER DEVELOPMENT OF IN VITRO CULTURED COTTON OVULES
HAFIZA MASOOMA NASEER CHEEMA1, ASIA KHATOON2, AFTAB BASHIR2* AND KAUSER A, MALIK3
Abstract
The outer epidermal layer of the cotton ovule may differentiate in vitro to form a mass of white fluffy fibers in an appropriate liquid medium. The in vitro cultured fibers provide a model for studying not only the cellular physiology and development mechanism but also generate a key resource for promoter evaluation in developing fibers. However, the potential uses of in vitro cultured ovules are restricted due to the frequent problem of bacterial and fungal contamination. In this study, the effect of Ampicillin, Gentamicin and Sodium Azide on cotton fiber development was observed at various concentrations (100 µg/mL, 50 µg/mL and 0.5 µg/mL respectively) which were added in the media as contamination controlling agents. The in vitro grown ovules were evaluated at 5
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