PJB-2010-444
OPTIMIZATION OF PROCESS PARAMETERS FOR THE BIOSYNTHESIS OF CELLULASES BY TRICHODERMA VIRIDE
SHAZIA KANWAL MALIK1, HAMID MUKHTAR1, AMMAD AHMAD FAROOQI2 AND IKRAM-UL-HAQ1
Abstract
Present studies describe the optimization of process parameters for the production of cellulases by Trichoderma viride using submerged fermentation technique. The fermentation experiments were carried out in shake flasks using pretreated bagasse. Maximum production of cellulases (CMCase 1.57 U/ml/min, FPase 0.921 U/ml/min) was observed after a fermentation period of 72 hrs at an incubation temperature of 30ºC. Initial pH of the culture medium was also optimized and a pH of 5.5 was found to support maximum growth and enzyme production (CMCase 1.66 U/ml/min and FPase 0.932 U/ml/min) by T. viride. Different inorganic nitrogen sources were evaluated for the production of cellulases and Ammonium sulphate was found to be the best. The enzyme production was further enhanced by carrying out fermentation experiments using 25 ml of culture medium in 250 ml flask inoculated with 4% conidial inoculum.
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